Abstract
Abstract With up to 5 lasers and 30 analysis parameters, the ZE5 flow cytometer is equipped for complex multiplex analysis of cells. Excellent fluorescence sensitivity (<100MESF values in FITC, PE and APC channels) enables detection of antigens expressed at low levels. The three scatter options; forward scatter (FSC), side scatter (SSC) and forward scatter small particle detector (FSCspd) provide optimal resolution of particles >0.3um to 50um in size. Furthermore, the option to set a dual trigger using any scatter or fluorescence channels allows for optimal background reduction. For this study, the ZE5 was used to evaluate size beads and mixed microbial populations, and to perform immunophenotyping of multiple cellular subsets in human blood using multi-color panels. Beads sized 0.3um and larger were discriminated from noise when using scatter or fluorescence as a trigger. Analysis of mixed microbial populations clearly identified the unique populations within the mixture. For immunophenotyping experiments, PBMCs from normal healthy donors were stained using a 14 color panel to identify T cell, B cell, NK cells, dendritic cells and monocyte sub populations. The panel data was compensated accurately on the ZE5 and each sub-cellular population was well identified.
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