Abstract
Bowel health is an important factor for duck rearing that has been linked to feed uptake and growth and death rates. Because the regulatory networks associated with acute stress-mediated injury in the duck gastrointestinal tract have not clearly elucidated, we aimed to explore potential miRNA-mRNA pairs and their regulatory roles in oxidative stress injury caused by transport stress. Here, 1-day-old mallard ducklings from the same breeder flock were collected and transported for 8 h, whereas the control group was not being transported. Various parameters reflecting oxidative stress and the tissue appearance of the intestine were assessed. The data showed that the plasma T-AOC and SOD concentrations were decreased in the transported ducklings. The intestine of the transported ducklings also displayed significant damage. High-throughput sequencing of the intestine revealed 44 differentially expressed miRNAs and 75 differentially expressed genes, which constituted 344 miRNA-mRNA pairs. KEGG pathway analysis revealed that the metabolic, FoxO signaling, influenza A and TGF-β signaling pathways were mainly involved in the mechanism underlying the induction of intestinal damage induced by simulated transport stress in ducks. A miRNA-mRNA pair, miR-217-5p/CHRDL1, was selected to validate the miRNA-mRNA negative relationship, and the results showed that miR-217-5p could influence CHRDL1 expression. This study provides new useful information for future research on the regulatory network associated with mucosal damage in the duck intestine.
Highlights
The negative effect caused by environmental stress has been increasingly recognized and studied
The intestine transcriptome was analyzed using an Illumina platform, and the gene expression level was represented by the expected number of fragments per kilobase of transcript sequence per million base pairs sequenced (FPKM), which is a normalized, quantitative metric that considers the effects of gene sequencing depth and the length of the reads used for gene expression analysis (Trapnell, Cole, et al, 2010)
77 differentially expressed genes (DEGs) were identified among the two groups, and these included and 32 upregulated and 45 downregulated genes in the treatment group (TG) compared with the control group (CG) (Fig 2A)
Summary
The negative effect caused by environmental stress has been increasingly recognized and studied. Transportation stress is complicated by various physical challenges, including fasting, dehydration, motion, crowding and high temperature [1]. An acute stress model can be established using transportation, which induces a marked increase in the level of reactive oxygen species (ROS) [2, 3]. The interaction between high concentrations of ROS and cell membrane. Analysis of miRNAs and their target genes associated with duck intestine caused by transport stress. Sciences Younger Top-Notch Talent Program (Grant Numbers Q2018021)
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