Analysis of microbial diversity of tea tree (Camellia sinensis (L.) O. Ktze.) sick rhizospheric soil using soil metaproteomic technology

Abstract

Soil metaproteomics technology was used to analyse and undertand the changes in microbial diversity in rhizospheric soil of Tieguanyin tea trees (Camellia sinensis (L.) O. Ktze.) of different ages. The results showed We identified 2911 protein spots in the rhizosphere soil of tea tree. These were derived from 388 species of microorganisms [331 species of bacteria (85.31 %) and 57 species of fungi (14.69 %)]. According to the analysis of microbial species in tea tree rhizospheric soil with different ages, the number of microbial species in 0, 4, 9 and 30-years old rhizospheric soils were 346, 350, 345 and 354, respectively. Among them, 310 microbial species co-existed in the rhizospheric soil with different ages tea trees. The analysis of results of microbial species and function showed that, as the tea tree age increased, the pathogenic microorganisms in its rhizospheric soil increased, while the microorganisms, for decomposing harmful substances, probiotics and microorganisms related to carbon cycle and nitrogen cycle decreased. The microbial structure and functions of tea tree rhizospheric soil changed significantly with increase in tea tree age.