Analysis of microbial community in a continuous flow process at gene and transcription level to enhance biological nutrients removal from municipal wastewater

Abstract

In biological municipal wastewater treatment, gene level analysis of community structure could not determine functional genes that actually played a role and expression of viable microorganism. In this study, reverse transcriptional PCR (RT-PCR), cDNA high throughput sequencing and transcriptional activity analysis were conducted to investigate active microbial community with nitrogen and phosphorus removal from municipal wastewater. RT-PCR and correlation heatmap analysis suggested that transcriptional activities of bacteria had strong correlation with performance of nitrogen and phosphorus removal, they might be therefore regarded as an indicator for wastewater treatment monitoring. When DO concentration were raised from 0.6 mg/L to 2 mg/L and C/N ratio from 3–4 to 5, the increase of population abundance and transcriptional activities of denitrifying genes improved the removal efficiencies of COD and TN. The species with relatively high abundance at gene level were not really active species at transcription level, and vice versa.