Abstract
Methylated purine bases from urine can be quantitated by capillary gas chromatography-mass spectrometry with selected ion monitoring. The method is based on a separation of the purines in urine using XAD-2 column chromatography and bonded-phase extraction methods. The extracted samples are derivatized with N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide and analyzed by capillary gas chromatography-mass spectrometry. The tert-butyldimethylsilyl derivatives of the purines showed excellent GC and GC/MS properties suitable for low-level detection in extracts of human urine as well as in urine extracts from animals treated with the methylating carcinogen N-nitrosodimethylamine. The oxidized purine bases 7-methyl-8-hydroxyguanine, 8-hydroxyadenine, and 8-hydroxyguanine were also detected in urine. To our knowledge, the latter two modified bases have not been previously reported in normal urine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
