Analysis of Methanolic Fruit Extract of Citrus aurantifolia Using Gas Chromatography-Mass Spectrum and FT-IR Techniques and Evaluation of Its Anti-bacterial Activity

Abstract

The objectives of this study were analysis of the secondary metabolite products and evaluation antibacterial and antifungal activity. Bioactives are chemical compounds often referred to as secondary metabolites. Twenty nine bioactive compounds were identified in the methanolic extract of Citrus aurantifolia. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Citrus aurantifolia revealed the existence of the Thieno[2, 3-c]furan-3-carbonitrile, 2-amino-4, 6-dihydro-4, 4, 6, 6, Furfural, 2-Vinyl-9-[3-deoxy-β-d-ribofuranosyl]hypoxanthine, 2-Myristynoyl pantetheine, 2, 5-Furandione, dihydro-3-methylene-, Cyclohexene, 1-methyl-4-(1-methylethenyl)-, (S)-, O-Acetyl-4-hydroxyproline, 1, 5, 5-Trimethyl-6-methylene-cyclohexene, Acetic acid, 2-(1-buten-3-yl)-2-nitro-, ethyl ester, Methyl 3-hydroxytetradecanoate, L-α-Terpineol, 4-Methyl itaconate Glycyl-D-asparagine, 2(3H)-Benzofuranone, hexahydro-7a-methyl-, 7-Oxa-2-oxa-7-thiatricyclo[4.4.0.0(3, 8)]decan-4-ol, Cholestan-3-ol, 2-methylene-, (3β, 5α)-, Formic acid, 3, 7, 11,-trimethyl-1, 6, 10-dodecatrien-3-yl ester, 7-epi-cis-sesquisabinene hydrate, 2, 5-Cyclohexadien-1-one, 3, 5-dihydroxy-4, 4-dimethyl-2-(1-oxo, Pyrrolidin-2-one-3β-(propanoic acid, methyl ester), 5-methylen, D-Fructose, diethyl mercaptal, pentaacetate, n-Hexadecanoic acid, 2H-1-Benzopyran-2-one, 5, 7-dimethoxy-, Dihydroxanthin, Oleic acid, Octadecanoic acid, Phorbol, 9-Octadecenamide, (Z)-and 9-Octadecenamide. Clinical pathogens were selected for antibacterial activity namely, Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumonia, and Pseudomonas eurogenosa. Citrus aurantifolia has maximum zone against Escherichia coli 5.66±0.21.