Analysis of Methamphetamine in Human Urine Using Ionic Liquid Dispersive Liquid-Phase Microextraction Combined with HPLC

Abstract

A specific and sensitive method using ionic liquid dispersive liquid-phase microextraction coupled with high-performance liquid chromatography was developed and applied in the analysis of methamphetamine in human urine. In this experiment, 1-butyl-3-methylimidazolium bis (trifluoromethyl) imide ([BMIM]TF2N) was selected as the extraction solvent and parameters affecting the extraction efficiency such as the volume of ionic liquid, percentage of dispersant, dissolving temperature, extraction time, sample pH, centrifuging time and salting-out effect have been investigated in detail. Under optimized conditions, good linear relationship of methamphetamine was obtained in the range 0.04–20.00 μg mL−1, the correlation coefficient (r) was 0.9987, the limit of detection and limit of quantitation were 0.01 and 0.04 μg mL−1, respectively, and the enrichment factor was 44. The results showed that the method was simple, time-saving, special, clean, sensitive and it could be applied in the analgesics analysis of forensic trace evidences and addicted cases.