Abstract
A method using solid-phase extraction (SPE) has been developed for analysis of mepivacaine in human serum. A procedure for isolation of mepivacaine and lidocaine (internal standard) from human serum by use of Chromosorb 104 (acrylonitrile–divinylbenzene polymer) as extraction adsorbent is described in detail. Analysis was performed by gas chromatography on an HP-INNOWax (cross-linked PEG) capillary column, with flame ionization detection, after splitless injection. Relative standard deviations ranged between 3.6 and 4.4 for a serum mepivacaine concentration of 0.5 μg mL−1 and between 4.7 and 5.9 for a concentration of 1 μg mL−1. Recoveries were approximately 95%. The method was applied in a stomatological clinic to healthy volunteers to whom anesthesia with mepivacaine was administered.
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