Abstract
The concentration of alkyl phosphotriesters induced in mammalian DNA by many carcinogens can be determined by measuring the mean sedimentation coefficient of the single strands before and after hydrolysis of the triesters in 0.5 m NaOH at 37°C for 1 h. Experiments show that the difference between hydrolyzed and unhydrolyzed DNA containing methyl phosphotriesters can be quantitatively determined using the technique of difference sedimentation. Theoretical analysis indicates that there is no requirement for the accurate matching of the meniscus positions but that differences in DNA concentration between the two solutions have to be avoided. Practical procedures for the analysis and the calculation of the results are discussed.
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