Abstract
A competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies specific for the synthetic plant growth regulator maleic hydrazide (MH) has been evaluated for application to the measurement of MH in two potato varieties. This ELISA method requires minimal sample preparation and much less time and effort than the current analytical procedure. The limit of detection of the homologous ELISA systems used was approximately 1 ppm MH. The effect of the matrix on the assay depended upon the potato variety, the method of sample preparation, and the antibody used for analysis. Variability of recovery of a 10 ppm spike was lowest within blanks and highest among samples, indicating excellent reproducibility of the method, but also suggesting significant sample variability. The data presented demonstrate the ability of the ELISA to measure MH in potatoes at levels typically found in domestic potatoes.
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