Analysis of lonidamine in rat serum and testis by high performance liquid chromatography

Abstract

A HPLC method for the determination of lonidamine in serum and testis, suitable for pharmacological studies in the rat and other mammals, has been developed. Briefly, 0.5 mL of serum or about 0.2 g of testicular tissue were extracted with ethyl acetate and evaporated to dryness under nitrogen. The residue was redissolved in methanol and an aliquot was injected onto a C18 column eluted with a mobile phase consisting of acetonitrile:water (51:49, v/v), containing 0.1% trifluoroacetic acid. The eluate was monitored at 230 nm with a sensitivity of 0.05 AUFS. By this method, the pharmacokinetics and the serum and testicular levels of the drug up to 120 h after the administration of one single dose (100 mg/kg body weight) of lonidamine to Sprague–Dawley rats have been studied. Results were highly variable, as previously reported, but a very good linear correlation was found between the serum and the testicular levels, suggesting that, in the rat, and possibly in the human, testicular levels could be estimated based on the serum concentrations. Copyright © 2001 John Wiley & Sons, Ltd. Abbreviations used: ACN acetonitrile AUFS absorbance units at full scale; bw body weight; CV coefficient(s) of variation; EA ethyl acetate; HPLC high performance units at full scale; F flurbiprofen; LND lonidamine; NRS normal rat serum; NRT normal rat testes; NRTC normal rat testicular cytosol; P peak area; QC quality controls; S serum; TC testicular cytosol; TFA trifluoroacetic acid.