Abstract
PurposeThis study aimed to investigate the profiles of messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) in peripheral blood samples collected from polycystic ovary syndrome (PCOS) patients. In addition, an in-depth bioinformatics analysis regarding the lncRNA-mRNA co-expression network was performed.MethodsHigh-throughput sequencing was used to measure the profiles of mRNAs and lncRNAs expressed in the peripheral blood samples isolated from six patients (three patients with PCOS and three normal women). In addition, five differentially expressed lncRNAs were chosen to validate the results of high-throughput sequencing by quantitative RT-PCR (qRT-PCR). Furthermore, a lncRNA-mRNA co-expression network was constructed using the Cytoscape software.ResultsA total of 14,276 differentially expressed mRNAs and 4,048 differentially expressed lncRNAs were obtained from the RNA-seq analysis of PCOS patients and healthy controls (adjusted q-value < 0.05, Fold change >2.0).The qRT-PCR results were consistent with the data obtained through high-throughput sequencing. Gene ontology (GO) and KEGG pathway analyses showed that the differentially expressed mRNAs were enriched in the chemokine signaling pathway. In addition, the analysis of the lncRNA-mRNA co-expression network of the chemokine signaling pathway showed the involvement of 6 mRNAs and 42 lncRNAs.ConclusionClusters of mRNAs and lncRNAs were aberrantly expressed in the peripheral blood of PCOS patients compared with the controls. In addition, several pairs of lncRNA-mRNAs in the chemokine signaling pathway may be related to PCOS genetically.
Highlights
As one of the most common endocrinopathies in women of reproductive age, polycystic ovary syndrome (PCOS) is characterized by oligo-anovulation, hyperandrogenism, insulin resistance and increased risks of long-term endometrial cancer, metabolic syndrome (1), type 2 diabetes (T2D), and cardiovascular diseases (2)
The expression levels of long noncoding RNAs (lncRNAs) and mRNAs in the peripheral blood samples isolated from three PCOS patients and three normal subjects were compared
21,566 lncRNAs, 57,567 mRNA and 34,948 coding transcripts were examined in the peripheral blood samples isolated from PCOS patients and normal subjects
Summary
As one of the most common endocrinopathies in women of reproductive age, polycystic ovary syndrome (PCOS) is characterized by oligo-anovulation, hyperandrogenism, insulin resistance and increased risks of long-term endometrial cancer, metabolic syndrome (1), type 2 diabetes (T2D), and cardiovascular diseases (2). In most PCOS studies, the samples were collected from experimental animals, human ovarian granulosa cells (4), whole ovaries (5), oocytes (6) and cumulus cells (7), which pay more emphasis on the local microenvironment of the ovary and revealed that many genes are associated with PCOS. LncRNAs have been found to exist in a stable form and are protected from endogenous RNase activity in tissues and body fluids, such as urine and blood (9). Recent studies have shown that lncRNAs may be associated with the pathogenesis of PCOS. C-Terminal binding protein 1 antisense (CTBP1-AS) was a novel lncRNA found to regulate androgen receptor (AR) activity (12), while lncRNA SRA might act as an important mediator of adiposity-related processes in PCOS
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