Abstract
This chapter describes analytical and preparative thin-layer chromatography (TLC) of degradation products of a model dimer of β-O-4 lignin substructure that is the most frequent interphenylpropane substructure in lignin, by a lignin-degrading basidiomycete. Analytical TLC is usually used as a rapid, simple, and easy method of preliminary analysis and is useful for making decisions about the conditions for preparative TLC, column chromatography, and high-performance liquid chromatography. Compounds are identified tentatively by the cochromatography with standards on a TLC plate. In preparative TLC, procedures which do not harm the separated substances are preferable to identify the zones. Accordingly, the use of fluorescent layers is recommended. The chapter discusses the identification of the separated components by gas chromatography-mass spectrometry.
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