Abstract
The most common histological type of thyroid cancer is papillary thyroid carcinoma (PTC). Radical resection of the thyroid gland is currently the recommended method of treatment. Almost 75% of thyroidectomies performed just for diagnostic purposes are benign. Thus, the confirmation of innovative and more precise noninvasive biomarkers holds promise for the detection of PTC, which may decrease the number of unnecessary thyroid lobectomies. In this work, using the droplet digital PCR (ddPCR) method, we have analyzed the level of five miRNAs (let-7a, let-7c, let-7d, let-7f, and let-7i) in the plasma of patients with PTC and compared them with those of a healthy control group to investigate whether miRNAs also have value in the management of PTC. Levels of four miRNAs, namely let-7a, let-7c, let-7d, and let-7f, were significantly higher in PTC patients than healthy controls. Thus, the analysis of circulating let-7 can be a useful tool and support the currently used methods for PTC diagnosis. However, our observation requires further research on a larger patient group.
Highlights
Over the recent decades, there has been an increase in the incidence of thyroid cancer worldwide [1]
In this study, using droplet digital PCR (ddPCR), we have examined the level of five miRNAs in the plasma of patients with Papillary thyroid carcinoma (PTC) and compared them with those of a healthy control group to investigate whether miRNAs have a value in the management of PTC
We compared the levels of five miRNAs in plasma, by ddPCR, between the PTC cases and healthy subjects
Summary
There has been an increase in the incidence of thyroid cancer worldwide [1]. Expression of circulating microRNAs (miRNAs) has been found as potential blood-based biomarker in the diagnosis of cancer [5]. The differential expression of miRNAs in the circulation of patients with cancer when compared with healthy controls has been documented by numerous studies, making miRNAs a promising noninvasive biomarker. This makes them hopeful diagnostic and therapeutic targets in deregulated pathways in cancer development. Applied relative quantification of circulating miRNAs by quantitative PCR (qPCR) is limited by the difficulty of finding reliable endogenous reference genes in the plasma or serum. The expression of let-7a, let-7c, let-7d, and let-7f has never been tested in plasma of PTC patients
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