Abstract
The acute toxicity of an organophosphate insecticide chlorpyrifos, on biochemical parameters of fish Gambusia affinis was evaluated under static condition. The sub lethal concentration of the insecticide chlorpyrifos was found to be 0.284 ppm indicating the high toxicity of the insecticide. Fishes were exposed to 1/10th of sub lethal (0.0284 ppm) and the alteration of enzyme activity in liver and kidney of fish Gambusia affinis were studied at 15, 30 and 45 days of exposure. A significant increase in LDH activity in both tissue Liver and Kidney and the inhibition of ATPase leads to decreased ATP breakdown and reduced the availability of free energy was observed.
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