Abstract
An enzyme—amperometric method is proposed for the analysis of total phenols and l-dopa; the method is based on the enzyme tyrosinase, which is immobilized in a Nylon membrane and coupled to an oxygen gas-diffusion amperometric electrode. The method was applied to the determination of total phenols in urine and to l-dopa in formulations and was evaluated as a promising alternative to currently adopted methods, e.g. to a classical spectrophotometric technique, chosen as a reference method.
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