Abstract
目的研究造血干细胞供者NK细胞表面的KIR3DL1表达水平。方法对92例造血干细胞供者采用序列特异性引物PCR、基因测序法进行KIR和HLA基因分型,流式细胞术检测KIR3DL1的表达,并分析不同KIR基因型、单倍型、KIR/HLA受配体模式组KIR3DL1表达水平的差异性。结果92例供者中KIR-A/A、Bx1、Bx2基因型的频率分别为46.74%(43/92)、18.48%(17/92)、9.78%(9/92);KIR-A、B1、B2、B3单体型频率分别为70.33%(128/182)、10.99%(20/182)、7.14%(13/182)、4.39%(8/182);KIR3DL1识别HLA-BW4/BW4、HLA-BW4/BW6、HLA-BW6/BW6配体的频率分别为13.79%、67.81%、18.39%(P<0.001)。在基因型KIR-A/A、KIR-A/B、KIR-B/B组KIR3DL1的中位表达水平分别为18.77%(3.11%~49.24%)、13.14%(1.70%~63.32%)、0.37%(0.20%~2.60%),两两比较差异均有统计学意义(P<0.05)。KIR-A/A组KIR3DL1的中位表达水平高于KIR-Bx基因型且着丝粒端2DL2阴性组[11.20%(3.50%~36.08%)](P=0.019)。KIR3DL1识别HLA-BW4/BW4纯合和HLA-BW4/BW6杂合组KIR3DL1中位表达水平[17.61%(1.40%~49.24%)]高于HLA-BW6/BW6纯合不识别组[10.60%(3.50%~18.56%)](P=0.006)。结论KIR3DL1的表达水平在不同KIR基因型、单倍型以及不同HLA配体组中均存在差异。
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