Abstract
1. 1. Rat reticulocytes were incubated with rat 125I-Tf- 59Fe under conditions inhibiting heme synthesis. 2. 2. Cytosol, prepared from the reticulocytes, was separated and analysed by gel filtration and Amicon Ultrafiltration. An iron-containing low molecular weight fraction derived from the cytosol was further analysed by HPLC size-exclusion chromatography and HPLC reversed phase chromatography. 3. 3. Conditions inhibiting heme synthesis and uncoupling the oxidative phosphorylations lead to a large increase in the Fe-containing low molecular weight fraction in the cytosol. 4. 4. The components in the low molecular weight fraction have an apparent molecular weight of 5500 Dalton as determined with HPLC size-exclusion chromatography. 5. 5. The low molecular weight fraction contained several iron chelating components like glycin, 1 2 cystine and citrate, but no specific iron-binding proteins, nucleotides or pyrophosphate.
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