Abstract
AbstractA technique for developing a selective and highly sensitive detection method was studied, which responds to particular ionogenic surfactants using a system of connecting the post‐column reaction detector with a high performance liquid chromatograph (HPLC).HPLC conditions used for the separation were: column packing and size, TSK‐LS410 (5µ) and 6 mm i.d. × 250 mm; mobile phase, a mixture of methanol, water, sodium perchlorate and phosphoric acid. A Technicon AutoAnalyzer II system was used as the post‐column reaction detector. Orange II was used to form the hydrophobic ion‐pair complexes for cationic and amphoteric surfactants, and Methylene blue for anionic surfactants.By this method, cationic, amphoteric and anionic surfactants were easily identified in the chromatograms. The analytical results of their homolog distributions were in good agreement with those obtained by conventional gas chromatographic (GC) methods.
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