Abstract
A reversed-phase high-performance liquid chromatographic (HPLC) method for the determination of insulin in commercially available preparations has been developed. The HPLC method previously used for the separation of various insulins was subject to interference due to the preservatives in the preparations, so this HPLC method was modified. Insulin in preparations was determined without interference by the preservatives by means of HPLC method on a Lichrosorb RP-18 column using a mixture of acetonitrile and 5 mM tartrate buffer (pH 3.0) (27 : 73, v/v) containing 0.15 mM sodium sulfate as a mobile phase at 50°C. The values obtained by the modified HPLC method were 95.0-108.8% of the labeled potency as the sum of potencies of both insulin and desamido-insulin, and the coefficients of variation were 1.03-3.14% (n=3).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
