Abstract
Capillary zone electrophoresis (CZE) has been combined with mass spectrometric detection for the separation and determination of inositol phosphates (IPs). Apart from IP1 through IP6 (inositol mono- through hexakisphosphate), an IP3 derivative has been analyzed and identified. The detection limits achieved are in the low micromolar range corresponding to an injected amount of ca. 900 fmol. In addition, an IP3 spiked plasma sample was analyzed after sample pretreatment using ultrafiltration.
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