Abstract
An indirect UV detection method based on capillary electrophoresis was developed to separate eleven metal cations completely, including alkali, alkaline earth and transition metal, which are related to evaluate the quality of honey. The background electrolyte contains 15 mmol/L chromophore imidazole, and acetic acid (pH = 3.7), which functioned as pH adjustor and complexing reagent. The selected cations can be completely separated within 8 min under hydrodynamic mode injection with a running voltage of 20 kV at 25°C. Limit of detection, linearity, reproducibility relative standard deviation of migration time and recoveries are in the range of 0.01-0.21 mg/L, 0.06-60.0 mg/L, 2.1-3.4% and 95.4-104.1%, respectively. The applicability of the method is shown by the analysis of honey samples, including a comparison with results of pretreatment and unpretreatment of eight samples. The results demonstrate that the developed method can conveniently be used in routine analysis of honey.
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