Analysis of Inorganic Anions by Capillary Zone Electrophoresis.

Abstract

Determination of inorganic anions by capillary zone electrophoresis is reported in this study. An anions mixture of bromide, chloride, fluoride, nitrite, sulfite, sulfate, perchlorate, chlorate and chlorite was separated by a capillary column and detected by indirect UV method. The running buffer contained 5mM ammonium dichromate, 10mM ammonium acetate, 20mM diethylenetriamine, and in 10% methanol solution at pH 9.3. A potential of 15kV at the cathode(reversed polarity) was utilized for the separation of inorganic anions. A complete separation of anions was achieved in less than 10 min and the applicabilities of the method were demonstrated for forensic evidences. We performed analysis of inorganic anions in toluene inhaled human's urine and in postmortem bloods by capillary zone electrophoresis and ion chromatography