Analysis of influence factors of alanine transaminase in voluntary blood donors in Chengdu

Abstract

Objective To explore the influence factors of unqualified alanine aminotransferase (ALT) of voluntary blood donors in Chengdu. Methods From June 1, 2012 to June 1, 2013, a total of 16 424 voluntary blood donors from a street blood collection point of Chengdu Blood Center were selected as subjects by random sampling method. Among them, there were 8 860 male and 7 564 female donors, aged from 18 to 55 years. Blood samples for ALT primary screening were detected by dry chemical method, and blood samples for ALT laboratory re-examination were detected by rate method. The ALT value > 40 U/L was determined to be unqualified for ALT blood screening or laboratory re-examination of blood donors. The information management system of Tangshan Qiao 5.0 was used to collect the data of blood donors including gender, age, height, weight, marital status, nationality, occupation, educational level and the seasons of blood donation. The ALT unqualified rates of blood donors with different demographic characteristics (gender, age, marital status, nationality, occupation and education level), seasons of blood donation and body mass index (BMI) were calculated respectively. Chi-square test was used to compare the ALT unqualified rates of blood donors with different demographic characteristics, seasons of blood donation and BMI. Multivariate unconditional logistic regression analysis was used to further study the independent influence factors of ALT disqualification in voluntary blood donors. The procedures followed in this study were in accordance with the requirements of the World Medical Association Declaration of Helsinki revised in 2013. And the Informed Consent of Blood Donors were signed with all blood donors before blood donation. Results ① Among the 16 424 voluntary donors in this study, and the unqualified rate of ALT was 14.4% (2 363/16 424). Among them, a number of 2 256 donors failed in ALT primary screening, accounting for 95.5% (2 256/2 363); and 107 donors failed in ALT laboratory re-examination, accounting for 4.5% (107/2 363). ② The results of univariate analysis for the influence factors of ALT disqualification in 16 424 voluntary blood donors in this study showed that there were significant differences in ALT unqualified rates among donors of different gender, age, marital status, occupation, education level, seasons of blood donation and BMI, respectively (χ2=872.65, 627.76, 575.94, 641.88, 150.64, 50.71, 2 152.05; P<0.001). ③ The results of multivariate unconditional logistic regression analysis showed that male (OR=3.52, 95%CI: 3.13-3.96, P<0.001), age of 26-35 years (OR=1.25, 95%CI: 1.07-1.46, P<0.001), 18.5 kg/m2 ≤ BMI<25.0 kg/m2 (OR=2.34, 95%CI: 1.24-4.45, P=0.010), 25.0 kg/m2≤BMI<28.0 kg/m2 (OR=7.51, 95%CI: 3.98-14.29, P<0.001) and BMI≥28.0 kg/m2 (OR=19.48, 95%CI: 10.13-37.45, P<0.001); married (OR=1.27, 95%CI: 1.09-1.47, P<0.001); blood donation in summer (OR=1.50, 95%CI: 1.26-1.79, P<0.001), blood donation in autumn (OR=1.79, 95%CI: 1.51-2.13, P<0.001), blood donation in winter (OR=1.27, 95%CI: 1.02-1.47, P<0.001) were the independent risk factors for ALT disqualification in blood donors. Student donor was the independent protective factor for ALT disqualification of blood donors (OR=0.31, 95%CI: 0.25-0.37, P<0.001). Conclusions The ALT unqualified rate of blood donors in Chengdu is at a high level. Independent risk factors for ALT disqualification in blood donors included age of 26-35 years, BMI≥18.5 kg/m2, married, and blood donation in summer, autumn and winter. In view of these independent risk factors, corresponding measures should be taken to reduce the ALT unqualified rate of voluntary blood donors in Chengdu. Key words: Blood donors; Alanine transaminase; Population structure; Influence factors; Unqualified rate