Analysis of In Vivo Transcriptome of Intracellular Bacterial Pathogen Salmonella enterica serovar Typhmurium Isolated from Mouse Spleen.

Na Sun,Mengda Liu,Lanping Yu,Cong Liu,Yanying Song,Fangkun Wang

doi:10.3390/pathogens10070823

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important intracellular pathogen that poses a health threat to humans. This study tries to clarify the mechanism of Salmonella survival and reproduction in the host. In this study, high-throughput sequencing analysis was performed on RNA extracted from the strains isolated from infected mouse spleens and an S. Typhimurium reference strain (ATCC 14028) based on the BGISEQ-500 platform. A total of 1340 significant differentially expressed genes (DEGs) were screened. Functional annotation revealed DEGs associated with regulation, metabolism, transport and binding, pathogenesis, and motility. Through data mining and literature retrieval, 26 of the 58 upregulated DEGs (FPKM > 10) were not reported to be related to the adaptation to intracellular survival and were classified as candidate key genes (CKGs) for survival and proliferation in vivo. Our data contribute to our understanding of the mechanisms used by Salmonella to regulate virulence gene expression whilst replicating inside mammalian cells.

Highlights

Salmonella is a Gram-negative pathogen that poses a health threat to humans and livestock
Among the more than 2600 documented serotypes, Salmonella enterica serovar Typhimurium is the predominant serotype associated with salmonellosis worldwide [1,2]
A total of 1340 differentially expressed genes (DEGs) were screened through quantitative gene analysis and subjected to various analyses based on gene expression levels

Summary

Introduction

Salmonella is a Gram-negative pathogen that poses a health threat to humans and livestock. Typhimurium must adjust and adapt to rapidly changing intracellular and extracellular environments by downregulating the expression of non-essential genes, upregulating the expression of the genes necessary for survival, and expressing essential virulence genes [4,5,6,7]. Many of these adaptive processes are regulated at the transcriptional level [8]. In studies investigating the pathogenesis of Salmonella, the induction of highly expressed genes in vivo is a research focus [18,19,20,21]

Methods

Results

Conclusion