Abstract
Background: Natural killer (NK) cells are a type of cytotoxic lymphocyte. It is revealed that hyperthermia which is used in cancer treatment, increases natural killer cell activity. In this study, our aim was to analyze effects of in-vitro hyperthermia on human Natural Killer cytotoxicity against SW-872 cell line. Materials and Methods: In this study, we used SW-872 liposarcoma cell line as a target cell. Peripheral blood NK cells were isolated from normal individuals by MACS. NK cells were treated at 39°C for 1hr .The expression of CD69 on the surface of NK cells was examined by flow cytometry at different time points including 0, 6 and 12 hrs. NK cell cytotoxicity was measured by LDH assay 12hrs after co-culture with SW-872. The results were compared to the conditions that both NK cells and SW-872 cells were heat treated at 39°C for 12hrs. Results: Our results revealed that cell killing activity of NK cells treated with heating alone was increased 6hrs after heat treatment at 39°C compared with heat-treated NK-target cells co-culture. While in heat-treated NK-target cells co-culture the maximum cytotoxicity was observed 12hrs after heat treatment at 39°C. Conclusion: These results showed that thermotherapy could be a feasible method to stimulate immune response against tumor cells. [GMJ.  2015;4(3):75-81]
Highlights
Hyperthermia is a type of cancer treatment in which body tissue is exposed to high temperatures to damage and kill cancer cells
It is revealed that hyperthermia which is used in cancer treatment, increases natural killer cell activity
Natural killer (NK) cells were treated at 39°C for 1hr .The expression of CD69 on the surface of NK cells was examined by flow cytometry at different time points including 0, 6 and 12 hrs
Summary
Hyperthermia is a type of cancer treatment in which body tissue is exposed to high temperatures (up to 113°F) to damage and kill cancer cells. The aim of this study is to investigate the killing ability of NK cells after hyperthermia .NK cells are heated for one hour at temperatures 39°C and 42 °C. The cytotoxicity of these cells in the co-culture with liposarcoma cells line (SW-872) after 0, 6 and 12 hours was evaluated with LHD-release assay kit. It is revealed that hyperthermia which is used in cancer treatment, increases natural killer cell activity. Our aim was to analyze effects of in-vitro hyperthermia on human Natural Killer cytotoxicity against SW-872 cell line.Materials and Methods: In this study, we used SW-872 liposarcoma cell line as a target cell. Conclusion: These results showed that thermotherapy could be a feasible method to stimulate immune response against tumor cells. [GMJ. 2015;4(3):75-81]
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