Abstract
The major active form of human thrombin, α-thrombin, was analyzed by hydrophobic interaction high-performance liquid chromatography (HIC-HPLC). The chromatographic system included a polymeric phenyl column and elution was performed by a gradient, 2–0 M sodium chloride (5–20 min). Total analysis time was 30 min per injection. By this method, a good resolution between α-thrombin and the proteolytically modified thrombin forms, β- and γ-thrombin, was obtained. In addition, the thrombin preforms, prothrombin, prethrombin 1, and prethrombin 2, were also resolved from α-thrombin in the system. The results from the HIC method were compared to those obtained from non-reducing sodium dodecyl sulfate–polyacrylamide gel electrophoresis. By this high-resolution chromatographic method, the rapid analysis of purified α-thrombin is possible.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
