Abstract
BackgroundThough sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; however, it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality.MethodsAfter whole blood collection, human synovium and bone marrow were harvested. Nucleated cells were expanded with autologous human serum and FBS.ResultsHeat-inactivation of autologous human serum enhanced proliferation of synovial MSCs. Heat-inactivation of each types of serum didn't affect calcification of synovial MSCs. The induction of calcification increased ALP activity, with the exception of bone marrow MSCs with autologous human serum. For adipogenesis of synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS.ConclusionThese clarified the processing of human autologous serum and the influence of different sera for differentiation of synovial and bone marrow MSCs.
Highlights
Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown
In bone marrow MSCs, colony formation was hardly observed in culture with autologous human serum, which made it difficult to compare the efficiency of calcified-colony formation between the two serums, though autologous human serum appeared to reduce the Alizarin Red positive colony forming efficiency (Fig. 2A-C)
Adipogenic potential of human synovial and bone marrow MSCs cultured with autologous human serum or fetal bovine serum (FBS) In synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS
Summary
Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality. Mesenchymal stem cells (MSCs) are useful for regenerative medicine because of their significant proliferation and multipotential abilities. Increasing the safety of medical treatments with MSCs requires the use of autologous human serum [3]. Autologous human serum significantly increased the proliferation of synovial MSCs; in contrast, compared with fetal bovine serum (FBS), human serum decreased the proliferation of bone marrow MSCs [4]. Heat-inactivation drastically changes protein contents [7]; the influences of heat-inactivation of human autologous serum as well as FBS on MSC proliferation and multipotentiality are hardly known
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