Abstract
To investigate whether hTERT mRNA expression could be used as a diagnostic and prognostic marker of bladder transitional cell carcinoma. SYBR green I real-time fluorescence quantitative PCR method was used to quantify hTERT mRNA expression in exfoliated cells from patients with bladder transitional cell carcinomas. A significant correlation was observed between the level of hTERT mRNA expression in exfoliated cells collected in urine with pathological grade and clinical stage. Our results show that SYBR green real-time fluorescence quantitative evaluation of hTERT mRNA could be a useful marker of tumor progression for the early diagnosis and prognosis of bladder cancers.
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