Analysis of HLA class II and TAP alleles in Japanese patients with psoriasis vulgaris

Abstract

We investigated HLA class II and transporter associated with antigen processing (TAP) alleles in eighty-five unrelated Japanese patients with psoriasis vulgaris and fifty-two healthy controls using the polymerase chain reaction-restriction fragment length polymorphism method. The frequencies of DRB1∗1502 and DQB1∗0601 were increased in the patient group (DRB1∗1502; 21% vs 12%, p < 0.05, DQB1∗0601; 35% vs. 21%, p < 0.05), while the frequencies of DRB1∗0406 and TAP2∗E were decreased in the patients (DRB1∗0406; 2% vs 9%, p < 0.05, TAP2∗E; 4% vs 11%, p < 0.05). However, none of these remained significant after p values were corrected for the number of comparisons made ( pc > 0.05). We also analysed specific amino acids on HLA class II molecules, but no significant difference was found between the two groups. Our previous reports clarified that aspartate at residue 9 (48% vs 20%, p < 0.002) and alanine at residue 73 (81% vs 48%, p < 0.0001) on HLA-C molecules were strongly associated with Japanese patients with PsV. These specific amino acids on HLA-C molecules are supposed to play more important roles compared with HLA class II and TAP alleles in the development of psoriasis vulgaris.