Abstract
Developing precise and efficient methods of monitoring immune responses against cytomegalovirus (CMV) infection in immunocompromised transplantation patients is important. With the aim of optimizing the monitoring strategy, an HLA-A24-CMVpp65 tetramer-based analysis of CMVpp65 peptide-specific CTL lines was performed. Previously, the HLA-A24-restricted CTL epitope of CMVpp65 matrix protein was identified (QYDPVAALF aa 341–349). In the present study, CMVpp65 (aa 341–349) peptide-specific CTL lines were obtained from PBLs of 12 HLA-A24 + healthy donors by two stimulations with peptide-pulsed dendritic cells (DC). Among 12 CTL lines, 9 showed HLA-A *2402-CMVpp65 tetramer staining, which was found to be strongly co-related to the amount of IFN-γ produced by CMVpp65 peptide-restimulated CTL lines ( r = 0.943, P < 0.001). These results suggested that HLA-A *2402-CMVpp65 tetramer staining was an efficient way to monitor immune responses against CMV infection in HLA-A24 + immunocompromised hosts.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
