Year-end Sale % OFF 
Abstract
Herpes simplex virus (HSV) DNA molecules synthesized in isolated nuclei in vitro were analyzed by centrifugation in sucrose gradients and by chromatography on benzoylated naphthoylated DEAE (BND)-cellulose columns. Some of the in vitro synthesized viral DNA molecules were found to have a high molecular weight when cosedimented with a HSV-DNA marker. Over 60% of the in vitro labeled DNA molecules were eluted from the BND-cellulose columns by 1.0 M NaCl containing 2% caffeine, indicating the presence of single-stranded sequences in the DNA molecules, a property of replicating DNA molecules. It is concluded that under in vitro conditions, the elongation of nascent viral DNA molecules takes place.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
