Abstract
BackgroundSubclinical necrotic enteritis (SNE) widely outbreaks in chickens which inflicted growth-slowing, causing enormous social and economic burdens. To better understand the molecular underpinnings of SNE on lipid metabolism and explore novel preventative strategies against SNE, we studied the regulatory mechanism of a potential probiotic, Lactobacillus johnsonii BS15 on the lipid metabolism pathways involved in chickens with SNE.MethodsOne hundred eighty one-day-old chickens were randomly divided into three groups and arranged with basal diet (control and SNE group). Added with BS15 (1 × 106 cfu/g) or Man Rogosa Sharpe (MRS) liquid medium for 28 days. The hepatic gene expression of each group was then measured using high-throughput analysis methods (RNA-Seq). Quantitative real-time PCR (qRT-PCR) was used to detect the expression changes of the related genes.ResultsThe results showed that there are eleven lipid metabolic pathways were found during the prevention of BS15 treatment in SNE chickens by RNA-Seq, including the peroxisome proliferator-activated receptor (PPAR) signaling pathway and arachidonic acid metabolism. BS15 notably facilitated the expressions of fatty acid binding protein 2 (FABP2), acyl-CoA synthetase bubblegum family member 1 (ACSBG1), perilipin 1 (PLIN1) and perilipin 2 (PLIN2), which were involved in PPAR signaling pathway of SNE chickens. Besides, suppression of phospholipase A2 group IVA (PLA2G4A) in arachidonic acid metabolism was observed in SNE chickens after BS15 prevention. The expression patterns of FABP2, ACSBG1, PLIN1, PLIN2 and PLA24G in qRT-PCR validation were consistent with RNA-Seq results.ConclusionsThese findings indicate that SNE may affect the hepatic lipid metabolism of chickens. Meanwhile, BS15 pretreatment may provide a prospective natural prophylaxis strategy against SNE through improving the PPAR signaling pathway and arachidonic acid metabolism.
Highlights
Subclinical necrotic enteritis (SNE) widely outbreaks in chickens which inflicted growth-slowing, causing enormous social and economic burdens
We firstly discovered that Lactobacillus johnsonii BS15 (CCTCC M2013663) strain exhibited the beneficial effects on lipid metabolism in the previous studies, resulting in preventing non-alcoholic fatty liver disease in obese mice [13]
Genes and pathways related to lipid metabolism associated with SNE disease We analyzed alterations in lipid metabolism gene expression in three SNE and three normal chicken liver tissue specimens using the powerful RNA sequencing technology. 385 genes as being differentially expressed in the SNE liver samples as compared with control animals’ (p < 0.05) were observed (Fig. 2, Additional file 1: Table S1)
Summary
Subclinical necrotic enteritis (SNE) widely outbreaks in chickens which inflicted growth-slowing, causing enormous social and economic burdens. We firstly discovered that Lactobacillus johnsonii BS15 (CCTCC M2013663) strain exhibited the beneficial effects on lipid metabolism in the previous studies, resulting in preventing non-alcoholic fatty liver disease in obese mice [13]. We applied this strain to chickens and found similar conditions, including it improved the meat nutritional value through altering the fatty acid composition [14], and promoted growth performance and lowered fat deposition in broilers [15]. We demonstrated that the aberrations of lipid metabolism during subclinical Clostridium perfringens infection was obviously ameliorated after BS15 prevention through controlling the lipid deposits and fatty acid composition [6]
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