Abstract
Pollution that occurred in Pasuruan area’s watershed Wangi–Beujeng river, District of Beji, Indonesia has been initiated in 2007 and continues to this day. The activity was caused by many factors such as the industrial (I), household wastes (II) and agriculture wastes (III) as well as the erosion process.The aims of this study to analyzed the heavy metal pollution that occurs in the Wangi river flow and the effect of gill histology and antioxidant activity on gambusia fish. The research method of observation at river flow and sampling for laboratory test.Three sites were assigned for chemical sampling and tissue histologycal in this study. Fish ( Gambusia affinis , local name: Gatul) and water were used as indikatorfrom each site to determine of cadmium (Cd), lead (Pb) and mercury (Hg) concentration using Atomic Absorption Spectrophotometry (AAS). The study was indicated that the streams previously used by residents for daily activities. Results showed level of cadmium (Cd), lead (Pb) and mercury (Hg) in Gambusia affinis exceeded the permissible standard (0.01 ppm Cd;0.03 ppm Pb;0.001 ppm Hg)respectively. Based on histologycal, the tissue showed of damage ofchloride cell (CC) which was used in ion homeostasis process and heavy metal route in grill fish. While protease activities, CAT, MDA and peroxidasse was increased in each sampling area, with significant different (>0.95) between the three sampling sites Keywords : Chloride cell, Heavy metal, Toxicity, Wangi river
Highlights
Pollution of industry wastes will be accumulation of water pollutant [1], [2]
The chemical-physical characteristics of aquatic environment was listed in Table1, providing several water quality parameters, including dissolved oxygen (DO), temperature, pH, chemical oxygen demand (COD) and biological oxygen demand (BOD) [13]
Water pollution observation data based on change level BOD5, COD, DO, temperature, pH, Detergent and metal observation (Pb, Cd and Hg) is presented in Table1 and Table 2.The result of water quality from three different sampling areas was showed in Table 1.Level of DO, pH and temperature were in normal condition for the life of aquatic organism
Summary
Pollution of industry wastes will be accumulation of water pollutant [1], [2]. Its substance from industry wastes have within it heavy metal[3]. Usually from industry, household and agricultural activities [13], as is the case in the Wangi river. The aims of this study to analyzed the heavy metal pollution that occurs in the Wangi river flow and the effect of gambusia fish on gill histology and some of its antioxidant enzyme activity. Discover environmental condition in the flow of Wangi River, especially in 3 different sites there are industrial area, household area and agriculture area Those areas are suspected has a great contribution in degradation of environment quality in the Wangi River’s flow. The effects of environmental degradation will discovered through Gambusiafish by observed the gill histology, heavy metal accumulation and the influence on antioxidant activity
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