Analysis of GSH1-GSH3 Genes by Comprehensive Use of Bioinformatics Method in Tomato

Abstract

GSH is a ubiquitous gene family in animals and plants, which plays a key regulatory role in plant growth, development and stress response. In this study, three GSH genes are identified in the Phytozome database, and bioinformatics analysis of physicochemical properties, structural domains, promoter cis-acting elements and phosphorylation sites is performed. The results show that the numbers of introns and exons of tomato GSH1-GSH3 are quite different. Promoters cis-acting elements are analyzed in GSH gene promoters, which account for a large proportion of light-responsive, hormone-responsive and protein synthesis elements. Transcription factors based on different tissues group data analysis reveal that the expression of GSH genes is different in different tissue development processes. The highest expression sites of GSH1-GSH3 are in 1-cm fruit, GSH2 currant tomato fruit and roots five days after veraison. According to physicochemical properties, transmembrane structure and signal peptide, the study finds that GSH proteins are all hydrophilic proteins and do not have a transmembrane structure and signal peptides. Based on the data analysis of protein phosphorylation sites and glycosylation sites, it is found that the number of the two sites differ significantly between GSH proteins. By means of prediction of secondary and tertiary structures in proteins, it finds that the three GSH proteins have four secondary structures: α-helix, extended chain, β-turn and random coil, but the proportions are different, and the tertiary structures of the three are quite different. Based on the analysis of protein phylogenetic tree, it is believed that tomato and potato are closely related. According to the protein interaction network, it points out that there is a strong link between GSH1 and GSH3. Through the analysis of tomato GSH family system, this study is expected to provide a theoretical basis for further research on the biological function of GSH gene.