Abstract
Thein vitrotranscription system using extracts from animal tissues is one of the most powerful and convenient ways to investigate transcriptional regulation of tissue-specific genes when appropriate cultured cells are not available. In the present study, we established a protocol for preparing transcription-competent nuclear extracts from mouse brain. Transcription in the extract could mimicin vivotissue specificity qualitatively, suggesting that this technique is beneficial for studying transcriptional regulation of nervous system-specific genes. By using these methods, we analyzedcis-elements of the glial cell-specific mouse myelin basic protein gene.
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