Abstract
A new high performance liquid chromatographic procedure is described for the analysis of ginseng saponins. Ginseng saponins were separated on Lichrosorb NH2 column and anthraquinone-2,6-disulfonate (AQDS) solution was added to the column effuent. The effluent was passed through 1.5 m-PTFE capillary coiled around 10 W-UV lamp to reduce AQDS to highly fluorescent 9,10-dihydroxyanthracene-2,6-disulfonate which was detected by fluorescence detector. The detection limit for the ginsenoside Rg1 by this method was found to be about 350 ng, the dynamic linear range was 102 and the correlation coefficient of the calibration curve was 0.9999.
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