Analysis of genome-wide contacts of forum terminus in Drosophila S2 cells

Abstract

Stable contacts between the regions of nonhomologous chromosomes have long been known from earlycytogenetic studies [1–5]; however, their nature is stillnot sufficiently studied. In recent years, new techniques specifically designed for a more accurate analysis of threedimensional structures of the chromosomes in the nucleus and to identify chromosomalregions that are close enough to each other were developed [6, 7]. These methods ar e based on the fixation ofchromatin structures by treatment with formaldehyde,Cleavage of DNA with an appropriate restrictionenzyme, and subsequent ligation of the sticky ends ofadjacent DNA fragments. Elegant methods for subsequent truncation of recombinant DNA molecules,their amplification by PCR, and analysis of the products by deep sequencing were proposed. Thus, it ispossible to reveal all the possible interactions of aselected region of the genome with other areas.Previously, we have obtained data indicating thatthe expression of