Analysis of genetic diversity and population structure of Pleuronectes yokohamae indicated by AFLP markers

Abstract

Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity and variation of two populations of Pleuronectes yokohamae from China and North Korea. A total of 228 loci ranging in size were detected from 63 individuals, of which 139 were polymorphic. The number of bands per primer combination varied from 37 to 51 and the percentage of polymorphic bands per primer combination ranged from 43.14% to 72.97%.The proportion of polymorphic loci, the Nei's genetic diversity and Shannon genetic diversity index of China and North Korea populations were 55.09% and 55.71%, 0.1205 and 0.1917, 0.1370 and 0.2129, respectively. The results showed that the genetic diversity of these two populations was at the same level. Dominant gene frequency revealed that these two populations have the same genetic population structure. F-st value of the two populations was 0.0986 and AMOVA analysis indicated that 80.75% genetic variation came from individuals within populations, and there were also 19.25% genetic differentiation occurred between the two populations. There was significant genetic difference between two populations. Exact-p test showed there was no random genetic communication between two populations. The UPGMA and NJ trees based on AFLP data supported this result. (c) 2012 Elsevier Ltd. All rights reserved.