Abstract
In this study, 26 mother plants and their respective 26 regenerants (30th cycles sub-cultured plants) of potato interspecific somatic hybrids were examined for genetic and epigenetic changes by amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) markers, respectively. Eight AFLP primer pair combinations yielded 78 polymorphic bands out of 353 scorable bands. Cluster analysis based on the Jaccard’s similarity coefficient exhibited 100 % similarity between the mother plant and their regenerants, whereas eight MSAP markers detected epigenetic variation (2–6 %) in the regenerants. Out of total 6411 MSAP sites in the regenerants, 6133 unmethylated, 132 (2 %) fully methylated, and 143 (2.2 %) hemi-methylated sites were detected. This study shows that a minimum epigenetic variation (2–6 %) was induced in the regenerants as compared to the mother plants of potato somatic hybrids during tissue culture process.
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