Analysis of Gene Expressions Associated with Response of Phenolic Metabolism of Allelopathic Rice under Barnyardgrass Stress

Abstract

Allelopathic rice varieties Giza 179 and Sakha 103 were induced by exogenous salicylic acid (SA) to suppress barnyardgrass weed (BYG) and were investigated using suppression subtractive hybridization (SSH) and real-time fluorescence quantitative PCR (qRT-PCR) methods. The results explained that exogenous salicylic acid could induce the allelopathic effect of rice on BYG. The variety Giza 179 showed higher inhibitory effect than Sakha 103 variety on BYG after treated with different concentrations of salicylic acid. Fourteen genes induced by salicylic acid were obtained by SSH analysis from Giza 179 variety. These genes encode ubiquitin carrier proteins, proteins related to phenyl-propanoid metabolism, receptor-kinase proteins, antioxidant related proteins and some growth-mediating proteins. The variations among gene expressions were validated by qRT-PCR in the two rice varieties. Previous studies indicated that cytoplasmic male sterile lines IR69625A, and G46A had general combining ability for panicle weight and grain yield per plant, while the varieties Giza 179 and Giza 178 had general combining ability for grain yield per plant and seed set percentage. In addition, with regard to the variety Giza 179 which have allelopathic potential and restoring fertility genes, so we recommend by using the variety Giza 179 as parental line with the used cytoplasmic male sterile line to produce hybrid rice have highly yield and strong allelopathy