Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)

Tao Yu,Shuaishuai Kan,Zhenhua Wang,Xiaochun Jin,Xianjun Liu

doi:10.1016/j.ejbt.2014.07.006

Tao Yu, Shuaishuai Kan + Show 3 more

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https://doi.org/10.1016/j.ejbt.2014.07.006

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Abstract

article i nfo Background: Head smut of maize, which is caused by Sporisorium reilianum f. sp. zeae (Kuhn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~14,850 probes was used to monitor the gene expression profiles between a disease resistant near isogenic line (NIL) and a highly susceptible inbred line after S. reilianum was injected with an artificial inoculation method. Results: Levels of expression for 3,532 genes accounting for 23.8% of the total probes changed after inoculation. Gene Ontology analysis revealed that the differentially expressed genes participated in physiological and biochemical pathways. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that plant-pathogen interaction, natural killer cell mediated cytotoxicity and benzoxazinoid biosynthesis pathways play important roles in resistance to head smut. Three head smut resistance-related candidate genes, CLAVATA1, bassinosteroid insensitive 1-associated receptor kinase 1 and LOC100217307 with leucine-rich repeat (LRR) conserved domains were identified, each of which is in maize mapping bin 2.09, a region previously shown to include a major QTL for head smut resistance. Furthermore, LOC100217307 was validated by quantitative real-time (qRT)-PCR inferring that this gene may be involved in the resistance to head smut of maize. Conclusions: This study provided valuable information for cloning, functional analysis and marker assisted breeding of head smut resistance genes.

Highlights

Head smut of maize (Zea mays L.), caused by Sporisorium reilianum f. sp. zeae (Kühn), is a disease that occurs in many regions of North America, Australia, Asia, and southern Europe [1,2]
Genetic analyses have revealed that a form of maize resistance to S. reilianum is inherited as a quantitative
Near isogenic line (NIL) BC4F4 progeny L282 was derived from the BC4F2 with the susceptible background (Huangzao4) carrying the major resistance quantitative trait loci (QTL) from the resistant donor line (Qi319) in bin 2.09 using simple sequence repeat (SSR) marker-assisted selection

Summary

Introduction

Head smut of maize (Zea mays L.), caused by Sporisorium reilianum f. sp. zeae (Kühn), is a disease that occurs in many regions of North America, Australia, Asia, and southern Europe [1,2]. Head smut of maize (Zea mays L.), caused by Sporisorium reilianum f. Genetic analyses have revealed that a form of maize resistance to S. reilianum is inherited as a quantitative. Head smut of maize, which is caused by Sporisorium reilianum f. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~14,850 probes was used to monitor the gene expression profiles between a disease resistant near isogenic line (NIL) and a highly susceptible inbred line after S. reilianum was injected with an artificial inoculation method. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that plant–pathogen interaction, natural killer cell mediated cytotoxicity and benzoxazinoid biosynthesis pathways play important roles in resistance to head smut. Conclusions: This study provided valuable information for cloning, functional analysis and marker assisted breeding of head smut resistance genes

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