Abstract
Helicobacter pylori (H. pylori) is one of the main causes of gastric gancer. TNF-related apoptosis-inducing ligand (TRAIL) is a protein able to promote apoptosis in cancer cells, however not in gastric cancer, which presents resistance to apoptosis via TRAIL. It is believed that MicroRNA-106b-5p might be involved in this resistance, although its role in Gastric Cancer is unclear. We aimed to determine the expression of microRNA-106b-5p and TRAIL in patients with gastric diseases, infected by H. pylori, and understand the relationship between these genes and their role in apoptosis and the gastric cancer pathways. H. pylori was detected by PCR, gene expression analysis was performed by real-time-qPCR, and bioinformatics analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cytoscape software. A total of 244 patients were divided into groups (Control, Gastritis, and Cancer); H. pylori was detected in 42.2% of the samples. The cancer group had a poor expression of TRAIL (p < 0.0001) and overexpression of microRNA-106b-5p (p = 0.0005), however, our results confirmed that these genes are not directly related to each other although both are apoptosis-related regulators. Our results also indicated that H. pylori decreases microRNA-106b-5p expression and that this is a carcinogenic bacterium responsible for gastric diseases.
Highlights
Gastric cancer is one of the most commonly diagnosed types of cancer—the fifth most frequent worldwide, and the third highest cause of cancer-related death—and for many years, it has been considered a global health problem [1,2,3,4]
Our results indicate that this bacterium is related to the occurrence of gastric diseases including gastric cancer, considering that it was prevalent in the groups of patients with gastric injury and rare in healthy patients
Our results demonstrate that H. pylori is a risk to the development of gastric disease, a result that is already described in the literature
Summary
Gastric cancer is one of the most commonly diagnosed types of cancer—the fifth most frequent worldwide, and the third highest cause of cancer-related death—and for many years, it has been considered a global health problem [1,2,3,4]. Helicobacter pylori (H. pylori) has been considered one of the main causes of gastric diseases, especially gastric cancer, considering that this Gram-negative bacterium successfully colonizes the stomach promoting intense inflammation and deregulating apoptosis process [6,7,8]. The apoptosis process can be activated by two pathways: the mitochondrial pathway (intrinsic pathway) and the death receptor pathway (extrinsic pathway) [10]. The latter pathway depends on the link between specific ligands that leads to cell death [12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
