Analysis of Frusemide and Spironolactone Mixtures Using First-derivative Spectrophotometry

Abstract

The aim of the present study was to demonstrate the feasibility and the possibility of derivative UV spectrophotometry for routine analysis of frusemide and spironolactone in combination formulations, simultaneously, to circumvent the problems of overlapping spectral bands. The method was applied to formulations available in the local market and compared with HPLC analysis of the two compounds to evaluate sensitivity, accuracy, precision and day-to-day variation. The proposed method was successfully applied for determination of the drugs in solid dosage forms. The method was rapid and cost-effective when compared with HPLC.