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Abstract
Test strips are convenient tools for rapid, semi-quantitative analysis of a variety of parameters by dipping them for a few seconds in a sample solution followed by a simple colorimetric read-out. Their sensitivity is mainly determined by the reactivity of the test dyes on the reaction zone and is not sufficient for some applications. The detection limit of commercially available free chlorine test strips, for example, is at present not low enough to confirm the absence of this analyte as disinfectant in rinsing solutions after disinfection or to control required residual amounts of chlorine in drinking water. Therefore, we developed a user-friendly lateral flow test which is capable to detect very low amounts of free chlorine. The latter relies on a larger sample volume passing the reaction zone as compared to simple dip test strips. An amount of as low as 0.05 ppm chlorine can, however, only be detected if oxidation stable flow test substrates are used. The eventually developed flow test reaches a 10x higher sensitivity than a commercial dip test. The result is obtained within 4–5 min flow time, whereby no action is required by the user during this analysis time.
Highlights
Test strips are convenient tools for rapid, semi-quantitative analysis of a variety of parameters by dipping them for a few seconds in a sample solution followed by a simple colorimetric read-out
In their simplest form, these devices consist of a plastic handle with a reaction zone composed of a few square millimeters of paper impregnated with specific test reagents
A free chlorine test containing leuco crystal violet (LCV) adsorbed on silica gel, which was described in the same patent, reached a detection limit of 0.2 ppm[9]
Summary
Test strips are convenient tools for rapid, semi-quantitative analysis of a variety of parameters by dipping them for a few seconds in a sample solution followed by a simple colorimetric read-out Their sensitivity is mainly determined by the reactivity of the test dyes on the reaction zone and is not sufficient for some applications. We developed a user-friendly lateral flow test which is capable to detect very low amounts of free chlorine The latter relies on a larger sample volume passing the reaction zone as compared to simple dip test strips. Test strips are mobile laboratories, which allow for semiquantitative analysis of ions, organic and inorganic substances anywhere by just dipping them for a few seconds in the sample solution In their simplest form, these devices consist of a plastic handle with a reaction zone composed of a few square millimeters of paper impregnated with specific test reagents. A flow test consisting of an oxidization stable substrate on which the dye is immobilized with cellulose fines or cellulose acetate, respectively
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