Abstract
By combined application of chemical pretreatments, and chromatographic and spectroscopic methods, it is possible to coordinate individual species of a complex lipid extract with high safety. In the sample studied, besides a high content of palmitic and cis-vaccenic acid, small amounts of unusual fatty acids containing hydroxy groups and cyclopropane rings were detected. The presence of polyunsaturated fatty acids was excluded by structure-retention relationships, which were obtained on phases of different polarity. On the basis of these studies, and without the use of reference substances, a routine analysis of these unusual fatty acids in biological materials is possible.
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