Abstract
Abstract CD38 is expressed in human T cells during early stages of activation and differentiation and can be used as a marker of prognosis for HIV. Currently, CD38 has raised interest since it has been found increased in patients with tuberculosis, however when the active phase is solved a decrease of CD38 in CD8 T lymphocytes is observed. Here we evaluated and determined the role of CD38 in human CD8 T lymphocytes stimulated with M. tuberculosis H37Rv soluble extracts (MTSE) and delipidized MTSE (dMTSE) in vitro. For this purpose, PBMC from healthy donors were incubated with PMA-ionomicyne, media, MTSE or dMTSE. After cultured, cells were harvested and stained with monoclonal antibodies conjugated to different fluorochromes. After kinetics experiments were performed and the expression of perforin, granzyme A, IFN-γ and CD38 in T CD8 cells was determined by flow cytometry. Expression of IFN-γ and perforin was statistically higher in T CD8+CD38+ in comparison with T CD8+CD38-. Remarkably, CD8+CD38+ T cells showed an increased cytotoxicity against macrophages pulsed with MTSE. These results suggest that the population of T CD8+ lymphocytes might have an effector functions in Ag specific dependance on CD38. This is the first report linking CTL activity to the CD38 molecule in tuberculosis.
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