Analysis of Expression Markers Intracellular Adhesion in Patients with Oropharyngeal Squamous Cell Cancer

Abstract

Objective. To learn the expression level of cell adhesion markers (Ecadherin and β�catenin) in oropharyngeal squamous cell carcinoma (OSCC) for establishing clinical and morphological connections and analyzing their prognostic meaning of relation with regional metastases. Design. Expression of Ecadherin and β�catenin, epi thelial cell adhesion molecules was examined by using immunohistochemical analysis (Ecadherin, clo n EP700Y, Lab Vision, β�catenin, clon E247, Lab Vision and visualization system EnVision, Dako Cytomation), both traditional histological parameters and the correlation of these markers with the histologically verified presence of regional metastases have been determined. Subjects. The study of 72 patients was comprised with OSCC. They were divided into two groups with regional metastases (T 3N1�3M0) � 38 patients and without metastases (T 3N0M0) � 34 patients. Paraffin� embedded material of primary tumors was used. Results. Obtained information is presented in the table of markersindexes expression. The difference of Ecadherin expression markers is established in gro ups with and without metastases. Differences between groups are significant statistically (p<0.001). The reliable indirect correlated connection has been established between these parameters (r=+0,471). The difference of β�catenin expression markers is established in groups with and without metastases. Differences between groups are significant statistically (p=0,002). The reliable indirect correlated connection has been established between these parameters (r=+0,373). It gives the opportunity to assert the practicability of Ecadhe rin and β�catenin markers usage to prognosis the me aning of relation with regional metastases. Conclusions. The expression levels of intercellular adhesion markers (Ecadherin and β�catenin) have been investigated with OSCC patients to identify clinical and morphological connections and analyze their prognostic meaning of relation with regional metastases. Markers relative predictive values of regional metastasis have been established. These results can be used for making decision on the subject of the patients` treatment of OSCC. Keywords: cancer, oropharyngeal squamous cell carcinoma, carcinoma, cell adhesion markers, Ecadherin, betacatenin, ephitelial cell adhesion molecule, im munohistochemical analysis, metastases, tumor, expression level, regional metastases, cancer treatment.