Abstract
BackgroundThe white-rot fungus Phlebia sp. MG-60 shows valuable properties such as high ethanol yield from several lignocellulosic materials, although white-rot fungi commonly degrade woody components to CO2 and H2O. In order to identify genes involved in ethanol production by Phlebia sp. MG-60, we compared genes differentially expressed by the ethanol producing fungus Phlebia sp. MG-60 and the model white-rot fungus Phanerochaete chrysosporium under ethanol fermenting and non-fermenting conditions using next-generation sequencing technologies.ResultsmRNAs from mycelia of Phlebia sp. MG-60 and P. chrysosporium under fermenting and non-fermenting conditions were sequenced using the MiSeq system. To detect differentially expressed genes, expression levels were measured in fragments per kilobase of exon per million mapped reads (FPKM). Differentially expressed genes were annotated using BLAST searches, Gene Ontology classifications, and KEGG pathway analysis. Functional analyses of differentially expressed genes revealed that genes involved in glucose uptake, glycolysis, and ethanol synthesis were widely upregulated in Phlebia sp. MG-60 under fermenting conditions.ConclusionsIn this study, we provided novel transcriptomic information on Phlebia sp. MG-60, and these RNA-seq data were useful in targeting genes involved in ethanol production for future genetic engineering.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2977-7) contains supplementary material, which is available to authorized users.
Highlights
IntroductionMG-60 shows valuable properties such as high ethanol yield from several lignocellulosic materials, white-rot fungi commonly degrade woody components to CO2 and H2O
All the results indicated that there exists a correlation between consumption of glucose and production of ethanol in the mycelia culture of Phlebia sp
MG-60, and investigated the genes involved in its high ethanol yield by comparing them to the model white-rot fungus P. chrysosporium
Summary
MG-60 shows valuable properties such as high ethanol yield from several lignocellulosic materials, white-rot fungi commonly degrade woody components to CO2 and H2O. Lignocellulosic biomass is mainly composed of cellulose, hemicellulose, and lignin and is the most abundant material for bioethanol production. Several white-rot fungi have been reported to ferment oligosaccharide materials to ethanol. Wang et al BMC Genomics (2016) 17:616 convert hexoses to ethanol [11], and Trametes hirsuta shows efficient fermentation of starch, wheat bran and rice straw to ethanol without acid or enzymatic hydrolysis [12]. Okamoto et al documented that the white-rot fungus Trametes versicolor KT9427 can produce ethanol from starch, cellulose, xylan, wheat bran and rice straw [13]
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