Analysis of Endonuclease activity in clinically and Environmental originated vibrio species.

Abstract

The research under given depicts the study vibrio species taken from faecal and environmental source. It is well known through researches that environment possess a very dense presence of cholera causing vibrio strains and that only a certain subsets of the strains are associated with human disease. The difference in the genetic profile of the clinical strains of the Vibrio species compared with their environmental counterparts was here exploited to trace specific human isolates to vectors of disease and also to allow a better estimation of whether a particular genotype is more often associated with disease or not. Isolation of Vibrio species was performed by taking water sample from river and Faecal matter they were cultured on TCBS Agar plate further they were screened through their biochemical properties. 6 different isolates were chosen to sonicate and bring up the lysates in order to obtain to assay their endonuclease activity, In every nook and cranny endonuclease activity was possessed by each and every isolate1_FS were in 13 fragments ,2 _WS in 10 fragments ,3_FS in 11 fragments. Thus it gave comparative assessment of Restriction endonuclease pattern.